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The Small Subunit RibosomalRNAgene (SSU rDNA) is a widely used tool to reconstruct phylogenetic relationships among foraminiferal species. Recently, the highly variable regions of this gene have been proposed asDNAbarcodes to identify foraminiferal species. However, the resolution of these barcodes has not been well established, yet. In this study, we evaluate fourSSU rDNAhypervariable regions (37/f, 41/f, 43/e, and 45/e) asDNAbarcodes to distinguish among species of the genusBolivina, with particular emphasis onBolivina quadratafor which ten new sequences (KY468817–KY468826) were obtained during this study. Our analyses show that a singleSSU rDNAhypervariable sequence is insufficient to resolve allBolivinaspecies and that some regions (37/f and 41/f) are more useful than others (43/e and 45/e) to distinguish among closely related species. In addition, polymorphism analyses reveal a high degree of variability. In the context of barcoding studies, these results emphasize the need to assess the range of intraspecific variability ofDNAbarcodes prior to their application to identify foraminiferal species in environmental samples; our results also highlight the possibility that a longerSSU rDNAregion might be required to distinguish among species belonging to the same taxonomic group (i.e. genus).